Application and Analysis of Biological Electrospray in Tissue Engineering

Abstract

Nanofiber scaffolds are suitable tools in tissue engineering. Electro spinning materials together with cells are not adequate to obtain a high cellular zing tissue construct as the shear force, tensile force, and other physical effects excited in the electro spinning process, which are harmful to cellular differentiation, development and function. However, this limitation has been overcome by a micro integration system containing bio-electro spraying human adipose stem cells (ASCs) and electro spinning Polyvinyl alcohol (PVA). Then, it was compared to the single electro spinning nanofiber scaffolds in relation to cell viability, which showed that the scaffolds through micro integration approach showed a larger number of surviving cells and is more suitable for cell growth and proliferation. In addition, the relationship between different parameters of biological electrospray (voltage, flow rate and distance of the needle from the collecting board) and droplet size of cell suspension was elucidated and the droplets with a near-mono distribution (<50um) could be generated to deposit a single living cell within a droplet. The association of bio-electro spraying with electro spinning (a scaffold preparation technique) has been demonstrated to be a promising and suitable tissue engineering approach in producing nanofiber based three-dimensional (3-D) cell seeded scaffolds.