RESEARCH ARTICLE


Evaluation of Madurahydroxylactone as a Slow Release Antibacterial Implant Coating



Muhammad Badar 1, Katherina Hemmen1, Manfred Nimtz1, Martin Stieve2, Meike Stiesch3, Thomas Lenarz 2, Hansjörg Hauser 1, Ute Möllmann 4, Sebastian Vogt 5, Matthias Schnabelrauch 5, Peter P Mueller*, 1
1 Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany
2 Department of Otolaryngology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
3 Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
4 Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knoell Institute, Beutenbergstrasse 11a, 07745 Jena, Germany
5 INNOVENT e.V., Prüssingstrasse 27 B, D-07745 Jena, Germany


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© Badar et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany; Tel: 0049 531 6181 5070; Fax: 0049 531 6181 5002; E-mail: pmu@helmholtz-hzi.de


Abstract

Madurahydroxylactone (MHL), a secondary metabolite with antibacterial activity was evaluated for its suitability to generate controlled drug release coatings on medical implant materials. A smooth and firmly attached layer could be produced from a precursor solution on various metallic implant materials. In physiological salt solutions these coatings dissolved within a time period up to one week. A combination of MHL with a broad spectrum fluoroquinolone antibiotic was used to create a coating that was active against all bacterial strains tested. The time period during which the coating remained active against Pseudomonas aeruginosa was investigated. The results indicated a delayed drug release from single layer coatings in the course of seven days. MHL was biocompatible in cell culture assays and could after a delay even serve as a cell adhesion substrate for human or murine cells. The findings indicate a potential for MHL for the generation of delayed release antimicrobial implant coatings.

Keywords: Antibacterial, biofilm, cell culture, cell proliferation, in vitro test, infection..