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Isolation and Characterization of an Aeromonas punctata Bacteriophage



Cheng Kai 1, 2, Zhang Denglan 1, Deng Jingxuan 2, Zhao Yijun 1, *
1 Key Laboratory of Ecological Remedlation of Lakes and Rivers and Algal Utilization of Hubei Province, College of Resources and Environmental Engineering, Hubei University of Technology, Wuhan, Hubei, 430068, P.R. China
2 College of Life Science, Central China Normal University, Wuhan, Hubei, 430079, P.R. China


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Creative Commons License
© Kai et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the College of Resources and Environmental Engineering, Hubei University of Technology, Hubei, 430068, P.R. China; E-mail: 1829088302@qq.com


Abstract

An Aeromonas punctata bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical Myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 PFU•Cell-1. Restriction enzyme pattern of the bacteriophage’s genome showed that the genome is a double-stranded DNA and about 34kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other Myoviridae bacteriophages at protein level.

Keywords: Myoviridae, bacteriophage, Aeromonas punctata.



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