RESEARCH ARTICLE
Macrophage Response to UHMWPE Submitted to Accelerated Ageing in Hydrogen Peroxide
Magda F.G. Rocha1, Alexandra A.P. Mansur1, Camila P.S. Martins2, Edel F. Barbosa-Stancioli2, Herman S. Mansur*, 1
Article Information
Identifiers and Pagination:
Year: 2010Volume: 4
First Page: 107
Last Page: 112
Publisher ID: TOBEJ-4-107
DOI: 10.2174/1874120701004010107
Article History:
Received Date: 8/2/2010Revision Received Date: 10/3/2010
Acceptance Date: 13/3/2010
Electronic publication date: 10/6/2010
Collection year: 2010
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
Ultra-high molecular weight polyethylene (UHMWPE) has been the most commonly used bearing material in total joint arthroplasty. Wear and oxidation fatigue resistance of UHMWPE are regarded as two important properties to extend the longevity of knee prostheses. The present study investigated the accelerated ageing of UHMWPE in hydrogen peroxide highly oxidative chemical environment. The sliced samples of UHMWPE were oxidized in a hydrogen peroxide solution for 120 days with their total level of oxidation (Iox) characterized by Fourier Transformed Infrared Spectroscopy (FTIR). The potential inflammatory response, cell viability and biocompatibility of such oxidized UHMWPE systems were assessed by a novel biological in vitro assay based on the secretion of nitric oxide (NO) by activated murine macrophages with gamma interferon (IFN-γ) cytokine and lipopolysaccharide (LPS). Furthermore, macrophage morphologies in contact with UHMWPE oxidized surfaces were analyzed by cell spreading-adhesion procedure using scanning electron microscopy (SEM). The results have given significant evidence that the longer the period of accelerated aging of UHMWPE the higher was the macrophage inflammatory equivalent response based on NO secretion analysis.