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RESEARCH ARTICLE

Characterization of Cells Interactions with Patterned Azopolymer-Based Materials using SEM, AFM and Video Microscopy

Régis Barillé1 Philippe Codron2 , 3 Guillaume Mabilleau4 Florence Manero4 Romain Mallet4 Sonia Zielinska5 Ewelina Ortyl5 Joël Eyer6 Franck Letournel3 , 6 , * Open Modal Authors Info & Affiliations
The Open Biomedical Engineering Journal • 30 Nov 2018 • RESEARCH ARTICLE • DOI: 10.2174/1874120701812010092

Abstract

Introduction:

Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.

Methods:

Here, we report a protocol for the use of a reconfigurable biocompatible azopolymer thin film through a photoinduced reconfigurable structuration of its surface to study nerve growth, differentiation and cell guidance.

Results & Discussion:

We show that this protocol combined with a spontaneous self-photoinduced polymer is suitable for time-lapse fluorescence video microscopy and can be easily adapted to electron microscopy techniques (SEM) and near-field imaging techniques (AFM).

Keywords: Polymer, PC12 cells, SEM, AFM, Time Laps, Video Microscopy.
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